Introduction to BLAST
BLAST is NCBI’s Basic Local Alignment Search Tool. A gene(s) or protein(s) can be aligned to a genome, collection of genes or collection of proteins. There are four BLAST programs.
There are four programs in blast that you can choose from.
| Query Type | Database Type | BLAST Program |
|---|---|---|
| Nucleotide | Nucleotide | blastn: Search a nucleotide database using a nucleotide query. |
| Nucleotide | Protein | blastx: Search protein database using a translated nucleotide query. |
| Protein | Nucleotide | tblastn: Search translated nucleotide database using a protein query. |
| Protein | Protein | blastp: Search protein database using a protein query. |
Index
More information
- Online BLAST
- Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J., 1990. Basic local alignment search tool. Journal of molecular biology, 215(3), pp.403-410.