Introduction

• Introduction

Experimental Design

• Experimental Design

Data Acquisition

• Introduction

File Transfer

• wget
• globus connect
• irods/iplant/cyverse

  SRAtoolKit

• SRAtoolKit (Downloading Raw Data)

Data wrangling

• Introduction

FASTA(Q) manipulations

• What is a Quality Score?
• Determine Sequence Lengths
• Converting FASTQ to FASTA
• Extract sequences based on sequence ID
• Microsoft Excel Tips and Tricks
• Create workbook from multiple text files
• Create text files from workbook with multiple worksheets
• Create Index for all worksheets

Data Analysis

• RNA-Seq

[Data Visualization]

[Appendix]