FastQCFastQC Report
Thu 14 Mar 2019
SRR2093871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2093871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022387
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC104090.1482259522296336Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG16200.034.9600144-45
GGTCGCC16050.034.84748544-45
GATCTCG18650.031.24922634-35
CTCGGTG19850.028.29481738-39
ATCTCGG21150.027.88879836-37
AGAGCGT43650.026.7032349
GCGTCGT21700.026.09908512-13
TCGGAAG48650.025.7943654
TGGTCGC21250.025.5459842-43
GAGCGTC22750.025.51420210-11
AGCGTCG23050.024.7743210-11
GTGGTCG22300.023.81623342-43
TCTCGGT24450.023.16352336-37
GATCGGA55250.023.1383842
AAGAGCG52200.023.0497328
TCGCCGT23550.022.95125646-47
CGCCGTA25050.022.51505546-47
GTGTAGG25800.022.4070616-17
AGATCTC26300.021.89156334-35
CGGAAGA57050.021.5845035
Produced by FastQC (version 0.11.3)