FastQCFastQC Report
Thu 14 Mar 2019
SRR2093871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2093871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022387
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT130110.18527888024399683TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA78180.11132966610925886TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA52550.049.988849
AGAGCAC61600.043.022468
CGTCTGA51350.036.4199616-17
ACGTCCG32350.036.103932-33
CACACGT52250.036.06550612-13
TCGGAAG74800.035.3676953
CCGCACA32700.035.3558736-37
CCAGTCA53250.034.59655826-27
CACGTCT56300.033.47109614-15
AGTCACG51250.033.33782628-29
GATCGGA80100.032.9672431
CGGAAGA80900.032.7587624
AGCACAC58350.032.6978510-11
GAAGAGC85750.031.1251186
GGAAGAG93050.029.087275
TGCCGTC55950.028.52029850-51
TCACGTC40900.028.50036830-31
ATCGGAA96650.027.585222
TCTGAAC68650.027.5504318-19
GCACATC41100.027.33271838-39
Produced by FastQC (version 0.11.3)